Potential of bovine kappa - casein as biomarker for detection of adulteration of goat’s milk with cow’s milk

Investigation of Goats’ Milk Adulteration with Cows’ Milk by PCR

Goats’ milk adulteration with cows’ milk is becoming a big problem. In the past, the urea-polyacrylamide gel electrophoresis assay with different motility of αS1-casein has been applied for the identification of cows’ milk adulteration. The detection sensitivity is 1.0%. The aim of this study was to develop a faster and more sensitive method to detect cows’ milk which may be present in adultera...

Fast biosensor immunoassays for the detection of cows' milk in the milk of ewes and goats.

Two monoclonal antibodies (MAb) raised against bovine kappa-casein were developed and applied in an automated optical biosensor (Biacore 3000) to create easy and fast direct and inhibition biosensor immunoassays (BIA) for the detection of cows' milk in the milk of ewes and goats. With both assay formats, low limits of detection (<01%) and fast run times (around 5 min) were obtained. For sample ...

Application of a duplex-PCR for detection of cows' milk in goats' milk.

A duplex-PCR method, with 2 pairs of primers recognizing sequences of mitochondrial D-loop region, was developed to identify cows' milk in the milk of goats. The PCR was shown to be specific and sensitive, enabling the detection of less than 1% of cows' milk added to the milk of goats. Simultaneous use of a primer pair for goats' and cows' mitochondrial DNA fragment prevented false negative res...

Adulteration of Milk

Adulteration of Human Milk

The necessity for testing human milk destined for milk banks in order to eliminate specimens which have been adulterated with water or cow's milk has been widely recognized since the introduction of such banks in 1910 (Mackintosh, 1947). In some centres, however, no tests for adulteration seem to be used, in spite of the fact that the mothers providing milk for these banks are not supervised be...